A late Anthony Dudley strike was in vain for Chester as they slipped to a 2-1 friendly defeat at Colne.
Blues managers Anthony Johnson and Bernard Morley named a strong line-up for the game against Northern Premier League hosts but were unable to build on Friday night's win at Cheadle Town.
Chester enjoyed the bulk of possession in the first half and had claims for a 25th minute penalty turned down after Dudley was felled inside the box.
Colne went ahead seven minutes before the interval when Sefton Gonzales met a cross into the box and his powerful header flew beyond Chester keeper Russ Griffiths.
That advantage was doubled early in the second half when Aidan Chippendale's cross was stabbed home by the Lancashire club's latest signing Tony Donaldson.
The visitors piled on the pressure as they sought a foothold in the game, with Danny Livesey and Jamie Morgan both hitting the woodwork for the visitors.
They finally reduced arrears in the 90th minute, when Dudley slotted home from the spot after the striker had been brought down.
Comments: Our rules
We want our comments to be a lively and valuable part of our community - a place where readers can debate and engage with the most important local issues. The ability to comment on our stories is a privilege, not a right, however, and that privilege may be withdrawn if it is abused or misused.
Please report any comments that break our rules.
Read the rules hereComments are closed on this article